Protein transduction by pseudotyped lentivirus-like nanoparticles
نویسندگان
چکیده
منابع مشابه
Production of Transgenic Pigs Mediated by Pseudotyped Lentivirus and Sperm
Sperm-mediated gene transfer can be a very efficient method to produce transgenic pigs, however, the results from different laboratories had not been widely repeated. Genomic integration of transgene by injection of pseudotyped lentivirus to the perivitelline space has been proved to be a reliable route to generate transgenic animals. To test whether transgene in the lentivirus can be delivered...
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Bone-marrow-derived mesenchymal stem cells (MSCs) have attracted considerable attention as tools for the systemic delivery of therapeutic proteins in vivo, and the ability to efficiently transfer genes of interest into such cells would create a number of therapeutic opportunities. We have designed and tested a series of human immunodeficiency virus type 1 (HIV-1)-based vectors and vectors based...
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Fig 2. GFP+ osteoclast. Lentivirus Transduction of Human Osteoclast Precursor Cells. Ramnaraine ML, + Clohisy DR, Mathews W +University of Minnesota, Minneapolis, MN. Senior author [email protected] INTRODUCTION Recent advances in understanding the pathophysiology of bone cancer have confirmed the pathologic role of osteoclasts [1]. In addition, the use of suicide gene delivery to tumors by acce...
متن کاملAltering α-dystroglycan receptor affinity of LCMV pseudotyped lentivirus yields unique cell and tissue tropism
BACKGROUND The envelope glycoprotein of lymphocytic choriomeningitis virus (LCMV) can efficiently pseudotype lentiviral vectors. Some strains of LCMV exploit high affinity interactions with α-dystroglycan (α-DG) to bind to cell surfaces and subsequently fuse in low pH endosomes. LCMV strains with low α-DG affinity utilize an unknown receptor and display unique tissue tropisms. We pseudotyped no...
متن کاملAssessment of F/HN-pseudotyped lentivirus as a clinically relevant vector for lung gene therapy.
RATIONALE Ongoing efforts to improve pulmonary gene transfer thereby enabling gene therapy for the treatment of lung diseases, such as cystic fibrosis (CF), has led to the assessment of a lentiviral vector (simian immunodeficiency virus [SIV]) pseudotyped with the Sendai virus envelope proteins F and HN. OBJECTIVES To place this vector onto a translational pathway to the clinic by addressing ...
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ژورنال
عنوان ژورنال: Gene Therapy
سال: 2011
ISSN: 0969-7128,1476-5462
DOI: 10.1038/gt.2011.38